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cell signaling technology 9101  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cell signaling technology 9101
    Cell Signaling Technology 9101, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16813 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+signaling+technology+9101/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+Antibody/pmc12852815-272-28-28
    Average 99 stars, based on 16813 article reviews
    cell signaling technology 9101 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Control:

    Article Title: Improved protein binder design using β-pairing targeted RFdiffusion.
    Article Snippet: .. Membranes were probed with the following primary antibodies: phospho-PDGFRα (Tyr762) (Cell Signaling Technology, #24188) 1:1000 dilution, Phospho-Akt (Ser473) (Cell Signaling Technology, #9271) 1:1000 dilution, Phosphop44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling Technology, #9101) 1:10,000 dilution, and either S6 (Cell Signaling Technology, #2117) at 1:1000 dilution or H3 (Cell Signaling Technology, #9715) at 1:5000 dilution as a loading control. .. After overnight incubation on a rocker, the membrane was probed with HRP-conjugated secondary antibody, washed 3 times, and imaged with a Bio-Rad ChemiDoc Imager.

    Article Title: Improved protein binder design using beta-pairing targeted RFdiffusion
    Article Snippet: .. Membranes were probed with the following primary antibodies: phospho-PDGFRα (Tyr762) (Cell Signaling Technology, #24188) 1:1000 dilution, Phospho-Akt (Ser473) (Cell Signaling Technology, #9271) 1:1000 dilution, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling Technology, #9101) 1:10,000 dilution, and either S6 (Cell Signaling Technology, #2117) at 1:1000 dilution or H3 (Cell Signaling Technology, #9715) at 1:5000 dilution as a loading control. .. After overnight incubation on a rocker, the membrane was probed with HRP-conjugated secondary antibody, washed 3 times, and imaged with a Bio-Rad ChemiDoc Imager.

    Article Title: Improved protein binder design using β-pairing targeted RFdiffusion
    Article Snippet: .. Membranes were probed with the following primary antibodies: phospho-PDGFRα (Tyr762) (Cell Signaling Technology, #24188) 1:1000 dilution, Phospho-Akt (Ser473) (Cell Signaling Technology, #9271) 1:1000 dilution, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling Technology, #9101) 1:10,000 dilution, and either S6 (Cell Signaling Technology, #2117) at 1:1000 dilution or H3 (Cell Signaling Technology, #9715) at 1:5000 dilution as a loading control. .. After overnight incubation on a rocker, the membrane was probed with HRP-conjugated secondary antibody, washed 3 times, and imaged with a Bio-Rad ChemiDoc Imager.

    Membrane:

    Article Title: Pattern-triggered immunity in blue and white seed cultivars of Papaver somniferum
    Article Snippet: 15 μg of total protein was separated by 12% SDS-PAGE and transferred to PVDF membrane (Immun-Blot Low Fluorescence PVDF Membrane, BioRad, 1620264). .. Membrane was blocked with 5% (w/v) bovine serum albumin (BSA) in Tris-buffered-salin (TBS)-0.1% (v/v) Tween-20 (T) (1 hour at room temperature, or overnight at 4 °C), incubated with primary antibody (Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody, Cell Signaling Technology, #9101; diluted 1:1000 in 1% (w/v) BSA in TBS-T, overnight at 4 °C), washed in TBS-T (5 times 5 minutes at room temperature), incubated with secondary antibody (StarBrightTM Blue 520 Goat Anti-Rabbit IgG, BioRad, #12005870; diluted 1:2500 in 1% (w/v) BSA in TBS-T, 30 minutes at room temperature), and finally washed in TBS (5 times 5 minutes at room temperature). .. The fluorescent antibody was detected with documentation unit ChemiDoc (BioRad) with the provided protocol for the detection of StarBright Blue 520.

    Incubation:

    Article Title: Pattern-triggered immunity in blue and white seed cultivars of Papaver somniferum
    Article Snippet: 15 μg of total protein was separated by 12% SDS-PAGE and transferred to PVDF membrane (Immun-Blot Low Fluorescence PVDF Membrane, BioRad, 1620264). .. Membrane was blocked with 5% (w/v) bovine serum albumin (BSA) in Tris-buffered-salin (TBS)-0.1% (v/v) Tween-20 (T) (1 hour at room temperature, or overnight at 4 °C), incubated with primary antibody (Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody, Cell Signaling Technology, #9101; diluted 1:1000 in 1% (w/v) BSA in TBS-T, overnight at 4 °C), washed in TBS-T (5 times 5 minutes at room temperature), incubated with secondary antibody (StarBrightTM Blue 520 Goat Anti-Rabbit IgG, BioRad, #12005870; diluted 1:2500 in 1% (w/v) BSA in TBS-T, 30 minutes at room temperature), and finally washed in TBS (5 times 5 minutes at room temperature). .. The fluorescent antibody was detected with documentation unit ChemiDoc (BioRad) with the provided protocol for the detection of StarBright Blue 520.

    Article Title: Cell wall integrity and elicitor peptide signaling modulate antimicrobial defense in Arabidopsis via jasmonic acid
    Article Snippet: .. Protein samples (35 μg total protein per lane) were separated on 10% SDS-PAGE gels and transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) at 25 V, 1.3 A for 7 min. Membranes were blocked in Tris-buffered saline with 0.1% Tween 20 (TBST) containing 5% (w/v) bovine serum albumin (BSA; Sigma-Aldrich, A3608) for 1 h, then incubated overnight with the primary phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (1:2000, Cell Signaling Technology, 9101). .. After three washes with TBST, membranes were incubated with the secondary HRP-linked anti-rabbit IgG antibody (1:6000, Cell Signaling Technology, 7074) for 2 h. Following three additional washes with TBST, chemiluminescence was detected using Clarity Western ECL Substrate (Bio-Rad, 1705061) for 2 min and visualized with the ChemiDoc imaging system (Bio-Rad).

    SDS Page:

    Article Title: Cell wall integrity and elicitor peptide signaling modulate antimicrobial defense in Arabidopsis via jasmonic acid
    Article Snippet: .. Protein samples (35 μg total protein per lane) were separated on 10% SDS-PAGE gels and transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) at 25 V, 1.3 A for 7 min. Membranes were blocked in Tris-buffered saline with 0.1% Tween 20 (TBST) containing 5% (w/v) bovine serum albumin (BSA; Sigma-Aldrich, A3608) for 1 h, then incubated overnight with the primary phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (1:2000, Cell Signaling Technology, 9101). .. After three washes with TBST, membranes were incubated with the secondary HRP-linked anti-rabbit IgG antibody (1:6000, Cell Signaling Technology, 7074) for 2 h. Following three additional washes with TBST, chemiluminescence was detected using Clarity Western ECL Substrate (Bio-Rad, 1705061) for 2 min and visualized with the ChemiDoc imaging system (Bio-Rad).

    Saline:

    Article Title: Cell wall integrity and elicitor peptide signaling modulate antimicrobial defense in Arabidopsis via jasmonic acid
    Article Snippet: .. Protein samples (35 μg total protein per lane) were separated on 10% SDS-PAGE gels and transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) at 25 V, 1.3 A for 7 min. Membranes were blocked in Tris-buffered saline with 0.1% Tween 20 (TBST) containing 5% (w/v) bovine serum albumin (BSA; Sigma-Aldrich, A3608) for 1 h, then incubated overnight with the primary phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (1:2000, Cell Signaling Technology, 9101). .. After three washes with TBST, membranes were incubated with the secondary HRP-linked anti-rabbit IgG antibody (1:6000, Cell Signaling Technology, 7074) for 2 h. Following three additional washes with TBST, chemiluminescence was detected using Clarity Western ECL Substrate (Bio-Rad, 1705061) for 2 min and visualized with the ChemiDoc imaging system (Bio-Rad).



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